Every lot of Thymosin Alpha 1 is tested by Freedom Diagnostics, an independent laboratory, for purity, identity and net content before release. Endotoxin and microbial results are added where those tests are run. Here is what each test measures, how results are reviewed, and how to check them yourself.
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No single method answers every question about a peptide sample. Thymosin Alpha 1 is tested with complementary methods so that each one covers what the others can't.
Reversed-phase high-performance liquid chromatography separates the sample's components by hydrophobicity, and a UV detector records each as a peak. Purity is the main peak's area as a percentage of all detected peak area.
Limits: does not identify the peak, and impurities that co-elute or absorb weakly in UV can be under-counted. More on HPLC
The separated sample enters a mass spectrometer via electrospray ionization, which gives the molecule one or more charges. The observed ions, near m/z 1555 (2+) and 1037 (3+), are matched against values calculated from Thymosin Alpha 1's molecular weight of 3108.32.
Limits: confirms molecular weight, not amino-acid order.
The laboratory quantifies the main compound in the tested vial against a standard. It is reported in milligrams next to the labeled vial size.
Limits: measured on the vial tested; other vials from the same lot are expected to be similar but are not individually measured.
The path every lot follows from production to a published certificate.
Thymosin Alpha Labs’ stated release specification is ≥95% HPLC-UV purity with identity confirmed by LC-MS. Current lots test at , .
Each lot carries a unique lot number (for example LOT123) printed on every vial label and on the certificate. The laboratory also assigns its own reference numbers (accession number and search code), which are listed on each lot page for cross-checking.
Certificates remain published in the archive after a lot sells through, so the documentation for any vial stays available.
Freedom Diagnostics is an independent, U.S.-based analytical testing laboratory. Its certificates for Thymosin Alpha 1 are signed by the laboratory's principal chemist and carry a QR code linking to the laboratory's own record.
Laboratory contact: freedomdiagnosticstesting.com, Admin@FreedomDiagnostics.net.
Certificate in progress. The current batch is with the testing laboratory. Its certificate of analysis will be published here, with purity, identity and net content, as soon as the report is issued. Current availability on Klow.bio.
| Ion | Calculation | Expected m/z | Observed on COAs |
|---|
Expected values are calculated by Thymosin Alpha Labs from the average molecular weight (3108.32 g/mol; H+ 1.0073, Na+ 22.99, K+ 39.10). Observed values are as printed on each lot's mass confirmation panel.
Thymosin Alpha 1 is analyzed with complementary methods: HPLC-UV for purity, LC-MS for identity, a quantitative measurement for net content, and, where listed, LAL endotoxin and PCR microbial testing. Results are published per lot at thymosinalpha.bio/coa/.
Every lot is tested by Freedom Diagnostics, an independent third-party analytical laboratory. Thymosin Alpha Labs does not test its own material for published certificates.
Purity is reported per lot by HPLC-UV.
By liquid chromatography–mass spectrometry (LC-MS), performed by Freedom Diagnostics on every lot. LC-MS separates the sample and measures the mass of the main component, confirming it has the molecular weight expected for the compound.
Endotoxin and microbial testing are reported per lot. Every lot page in the COA archive states whether those tests were performed.
HPLC (high-performance liquid chromatography) separates a sample's components so each appears as a peak. The purity percentage is the main peak's share of all UV-detected peak area. It does not confirm identity (the separate LC-MS test does) or measure the amount of peptide in the vial (net content does).
A Thymosin Alpha 1 certificate of analysis is the testing laboratory's report for one lot: the lot number, test dates, methods and results for purity, identity and net content. Thymosin Alpha Labs publishes every lot's COA as a readable page at thymosinalpha.bio/coa/.
Find the lot number printed on your vial label and enter it at thymosinalpha.bio/verify/, or open that lot in the COA archive at thymosinalpha.bio/coa/.
Scan the Freedom Diagnostics QR code printed on the certificate, then search for klow.bio under the Company tab to view the laboratory record.
Net content is the laboratory's measurement of peptide in the tested vial.
5 mg vials. The current batch's certificate is in progress. Current purchasing is available through Klow.bio.