Thymosin Alpha 1 specifications and molecular characteristics
The technical specification for Thymosin Alpha 1, the Thymosin Alpha 1 research material supplied by Thymosin Alpha Labs: molecular formula C129H215N33O55, average molecular weight 3108.32 g/mol, 28 residues, supplied as a white lyophilized powder. Each item is explained below.
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Specification table
| Product | Thymosin Alpha 1 |
|---|---|
| Type | Synthetic 28-residue acetylated peptide |
| Molecular formula | C129H215N33O55 |
| Molecular weight | 3108.32 g/mol (average) |
| Length | 28 residues |
| Sequence | Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN |
| N-terminus | Acetyl (Ac-Ser1) |
| C-terminus | Free acid (Asn28) |
| CAS number | 62304-98-7 |
| Form | White lyophilized powder |
| Vial sizes | 5 mg |
| Purity specification | ≥95% (HPLC-UV) |
| Identity specification | Confirmed by LC-MS |
| Testing laboratory | Freedom Diagnostics (independent) |
| Storage | Store sealed, dry and protected from light at the temperature stated on the vial label. Allow a cold vial to reach room temperature before opening. |
| Designation | Research use only |
Current lot results against specification
Thymosin Alpha Labs’ stated release specification is ≥95% HPLC-UV purity with identity confirmed by LC-MS. The current batch is with the testing laboratory. Its certificate of analysis will be published here, with purity, identity and net content, as soon as the report is issued.
Thymosin Alpha 1 molecular formula
The molecular formula of Thymosin Alpha 1 is C129H215N33O55. Includes the N-terminal acetyl group.
Thymosin Alpha 1 molecular weight
The average molecular weight of Thymosin Alpha 1 is 3108.32 g/mol. "Average" means it is calculated from natural isotope abundances, which is the value used to predict the ions seen in LC-MS. With this weight, Thymosin Alpha 1 is expected at m/z 1555.2 with two charges and m/z 1037.1 with three charges.
Thymosin Alpha 1 sequence
Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN
Structure and modifications
Ac-Ser1 is acetylated and Asn28 ends in a free acid. With no tryptophan, tyrosine or phenylalanine, the peptide absorbs only weakly above 220 nm, so HPLC purity is read at the peptide-bond wavelength.
Physical form
White lyophilized powder, recorded as the appearance on every certificate. Lyophilization (freeze-drying) removes water, which slows most chemical degradation. What lyophilized means.
Purity and identity specifications
Thymosin Alpha Labs’ stated release specification is ≥95% HPLC-UV purity with identity confirmed by LC-MS. The current batch is with the testing laboratory. Its certificate of analysis will be published here, with purity, identity and net content, as soon as the report is issued.
Analytical and testing methods
HPLC-UV for purity, LC-MS for identity and laboratory quantitation for net content, performed on every lot by Freedom Diagnostics. LAL endotoxin testing (USP <85>) and PCR microbial screening are reported for lots where they were run. Testing and quality control.
Research designation
Thymosin Alpha 1 is designated research use only: supplied for laboratory research by qualified personnel. Research use only policy.
Buy Thymosin Alpha 1
5 mg vials. The current batch's certificate is in progress. Current purchasing is available through Klow.bio.